ABSTRACT
Objectives@#The purpose of this study was to compare differences in facial soft tissue thickness in three-dimensional (3D) images before and after orthognathic surgery in patients with skeletal Class III malocclusion and to obtain a better understanding of the relationship between hard and soft tissue changes after surgery.Materials and method: The present retrospective study included 31 patients with skeletal Class III malocclusion with mandibular chin deviation greater than 4 mm who had undergone cone-beam computed tomography before and 6 months after surgery. Seven bilateral points were established. Measurements were taken from software-generated multiplanar reconstructions. The predictor variables were timing (pre- and postoperatively) and side (deviated vs.nondedicated). A regression model and correlation analysis were conducted for statistical analysis. @*Results@#The difference of bilateral facial soft tissue thickness was statistically significantly different between deviated and nondeviated sides (P < 0.05), with lower values observed on the deviated side. The soft tissue thickness has become nearly symmetric at local regions of the lower thirds of the face after orthognathic surgery. However, most measurements showed a negative correlation between changes in soft tissue thickness and changes in bone tissues. @*Conclusions@#Skeletal Class III malocclusion with facial asymmetry is accompanied by differences in soft tissue thickness when comparing Dev and N-Dev sides of the posterior region of the mandible, where soft tissues are thinner on the Dev side. Soft tissue thickness can compensate for or camouflage the underlying asymmetric mandible. In addition, the asymmetric soft tissue thickness on the lower third of the face can be partially improved by orthognathic surgery, but the amount of soft tissue thickness change is not consistent with that of hard tissue positional change.
ABSTRACT
Jatrogricaine A (1), a new diterpenoid possessing a 5/6/6/4 carbon ring system, together with eight known diterpenoids (2-9) were isolated from the stems of Jatropha podagrica. Their structures were elucidated by extensive spectroscopic methods and the absolute configuration of 1 was determined by single crystal X-ray diffraction analysis. All compounds were evaluated for their anti-inflammatory activities in vitro, and compound 3 showed significant inhibitory effects against nitric oxide (NO) production in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells with an IC of 13.44 ± 0.28 μmol·L, being comparable to the positive control, quercetin (IC 17.00 ± 2.10 μmol·L).
Subject(s)
Animals , Mice , Anti-Inflammatory Agents , Chemistry , Pharmacology , Carbon , Diterpenes , Chemistry , Pharmacology , Inhibitory Concentration 50 , Jatropha , Chemistry , Lipopolysaccharides , Toxicity , Macrophages , Metabolism , Molecular Structure , Nitric Oxide , Metabolism , Plant Extracts , Chemistry , Pharmacology , Plant Stems , ChemistryABSTRACT
Objective To assess the evaluation of SRT (Stereotactic radiotherapy ) and 3D-CRT (Three-dimensional conformal ra-diotherapy)on treatment of brain metastasis from lung cancer .Methods From June 2009 to June 2011 ,74 patients with multiple brain metastasis(brain metastasis ≤3 ,tumor mass ≤3 cm) from lung cancer were analyzed retrospectively ,37 patients received 3D-CRT alone were retrospectively compared with 37 patients who received SRT alone .the results was evaluated by median survival time(MS) ,local control(LC) and toxicity effect .Results The median survival time(MS) was 9 .3 ,which 8 .6 months after 3D-CRT ,and 10 .6 months after SRT .the local control rate was 73 .5% for 3D-CRT ,and 79 .6% for SRT after 3 months .there was no difference between two groups on toxicity effect from stastic .Conclusion The SRT was a efficacious methods for the treatment of brain metastasis from lung cancer ,which could improve the local control rate ,but there was no more toxiticy .
ABSTRACT
IQGAP1 is a scaffold protein which binds to various signaling and structural molecules.By interacting with target proteins,IQGAP1 participates in integration of signaling pathways and regulation of cellular functions.More and more evidences suggest that IQGAP1 plays an important role in tumorigenesis by promoting cell proliferation and transformation,weakening cell adhesion and stimulating cell motility and invasion.
ABSTRACT
Objective: To investigate the influence of secretory clusterin(sCLU) gene silencing by RNA interference on the proliferation, apoptosis and invasion of human ovarian carcinoma SKOV3 cell line. Methods: The human ovarian carcinoma SKOV3 cells were transfected with sCLU-targeting siRNA by Lipofectamine 2000. The study included four groups: the experimental group (sCLU-siRNA+Lipofectamine 2000), negative group I (negative control siRNA+Lipofectamine 2000), negative group II (sCLU-siRNA) and blank control group. The expressions of mRNA and protein were examined by RT-PCR and Western blotting analysis. The proliferation, apoptosis and invasion ability of SKOV3 cells were evaluated by MTT assay, flow cytometry and Transwell assay. Results: The sequence-specific siRNA of sCLU effectively and specifically down-regulated the expression of sCLU at both mRNA and protein levels. The MTT results showed that the proliferation of cells in the experimental group was significantly inhibited compared with those in the other groups (P < 0.05). Flow cytometry demonstrated that the apoptotic rate of cells in the experimental group([15.84±1.53]%) increased by 9% compared with those in the rest groups (P < 0.01). We also found that the matrigel penetrating capacity of cells in the experimental group ([26.52±6.22] cells/field) was significantly inhibited compared with those in the rest groups(P < 0.01). Conclusion: Silencing of sCLU can effectively inhibit the proliferation and invasion of human ovarian carcinoma SKOV3 cells, and it can also greatly promote their apoptosis, making sCLU gene a potential new target for ovarian carcinoma treatment.